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Table 5 The cytosolic proteome of H. mustelae determined by LC-MS

From: Comparative genomics and proteomics of Helicobacter mustelae, an ulcerogenic and carcinogenic gastric pathogen

Ranka Locus Annotation Mw Da MOWSE Scoreb Coveragec% emPAId Mol%
1 HMU14210 fldA flavodoxin 1 18,147 620 56 6.74 7.35
2 HMU05200 thiJ 4-methyl-5(beta-hydroxyethyl)-thiazole monophosphate synthesis protein 20,365 212 32 3.64 3.97
3 HMU01210 tpx probable thiol peroxidase 17,993 886 62 3.43 3.75
4 HMU12840 aroQ 3-dehydroquinate dehydratase 17,477 161 21 2.98 3.25
5 HMU12860 sodB superoxide dismutase (Fe) 24,576 534 45 2.41 2.63
6 HMU00320 undefined product 8,942 162 54 2.16 2.36
7 HMU05470 acpP acyl carrier protein 8,469 139 48 2.16 2.36
8 HMU03120 tuf elongation factor TU 43,570 1695 62 2.09 2.28
9 HMU12690 possible bacterioferritin 17,198 562 46 1.99 2.17
10 HMU09770 trxA thioredoxin 11,553 361 36 1.89 2.06
11 HMU04180 hypothetical protein Cj1613c 30,321 755 29 1.71 1.87
12 HMU03320 ahpC alkyl hydroperoxide reductase 22,088 572 44 1.68 1.83
13 HMU14370 cft ferritin 19,056 276 45 1.42 1.55
14 HMU00950 rpsA 30S ribosomal protein S1 60,196 208 7 1.31 1.43
15 HMU10800 lpsJ succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B 23,051 501 38 1.15 1.26
16 HMU04000 groEL 60 kD chaperonin (cpn60) 57,443 1474 48 1.02 1.11
17 HMU03150 rplK 50S ribosomal protein L11 12,586 473 48 0.90 0.99
18 HMU01260 ald alanine dehydrogenase 39,844 662 32 0.82 0.9
19 HMU07380 hupB DNA-binding protein HU homolog 10,122 352 56 0.81 0.89
20 HMU10120 peb4[1]f2 major antigenic peptide PEB3ll binding factor 2 31,816 750 39 0.77 0.85
21 HMU07050 rplI 50S ribosomal protein L9 16,251 460 49 0.74 0.81
22 HMU04730 oorB OORB subunit of 2-oxoglutarate:acceptor oxidoreductase 30,613 423 28 0.74 0.81
23 HMU14090 putative thioredoxin 11,796 267 39 0.730 0.8
24 HMU04740 oorA OORA subunit of 2-oxoglutarate:acceptor oxidoreductase 40,773 554 36 0.70 0.77
25 HMU02170 cheY chemotaxis regulatory protein 13,894 208 25 0.70 0.76
26 HMU11060 putative putative aminotransferase (nifS protein homolog) 43,365 424 26 0.66 0.73
27 HMU00290 fbp putative putative fructose-1,6-bisphosphatase 30,550 323 20 0.66 0.73
28 HMU03180 rplL 50S ribosomal protein L7/L12 12,946 259 44 0.66 0.73
29 HMU00100 putative putative acyl-CoA thioester hydrolase 16,960 168 20 0.63 0.7
30 HMU13330 undefined product 8,555 166 49 0.63 0.7
31 HMU03850 flaG possible flagellar protein 14,814 452 34 0.62 0.69
32 HMU03060 ureB urease alpha subunit 61,146 743 26 0.62 0.68
33 HMU09760 trxB thioredoxin reductase 33,972 587 35 0.62 0.68
34 HMU07390 ndk nucleoside diphosphate kinase 15,293 291 25 0.61 0.67
35 HMU05040 putative hydantoin utilization protein B 83,272 948 28 0.60 0.66
36 HMU10790 scoA succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A 25,076 305 32 0.58 0.64
37 HMU03990 groES 10 kD chaperonin (cpn10) 10,185 129 34 0.58 0.64
38 HMU13450 hypothetical protein Cj0706 27,047 694 49 0.55 0.61
39 HMU10960 katA catalase 52,490 781 39 0.53 0.59
40 HMU03670 aspA aspartate ammonia-lyase 51,394 620 31 0.51 0.57
41 HMU10290 rpoA DNA-directed RNA polymerase alpha chain 37,551 308 25 0.50 0.55
42 HMU11440 putative putative nucleotide phosphoribosyltransferase 17,378 164 25 0.50 0.55
43 HMU10630 putative putative periplasmic protein 21,100 282 24 0.48 0.53
44 HMU05030 putative putative hydantoinase A 78,444 681 24 0.47 0.52
45 HMU11200 glyA serine hydroxymethyltransferase 45,885 461 24 0.48 0.52
46 HMU08860 hypB hydrogenase isoenzymes formation protein 26,838 328 28 0.46 0.51
47 HMU01430 putative putative periplasmic cytochrome C 10,944 178 36 0.46 0.51
48 HMU06880 yabJ putative putative regulatory protein 13,506 163 26 0.46 0.51
49 HMU09820 surE SurE protein homolog 28,918 352 25 0.44 0.49
50 HMU12070 putative putative exported protein 16,551 270 33 0.44 0.49
  1. The 50 most abundant proteins are tabulated.
  2. a. Relative abundance ranked by mol%
  3. b. Score for the entire protein derived by MASCOT, and made up of the individual scores given to each peptide sequence
  4. c. Proportion of each protein sequence identified
  5. d. Exponentially modified protein abundance index. See Methods for details and reference.