Figure 1

Primer extension analysis of selected genes. Primer extension results are exemplified for selected promoters. Total RNA was isolated from MM139 or MC4100 cells. Different growth phases for RNA isolation are indicated by A: early log (A₆₀₀ = 0.4), B: late log (A₆₀₀ = 1.2), C: early stationary (A₆₀₀ = 2.7), D: stationary phase (A₆₀₀ = 3.3). The presence of functional 6S RNA is indicated by -- or +, respectively. The predominant sigma factor specificity is indicated in brackets below the respective genes. Note that rpoD P3 is a minor promoter, which we have assigned as σ⁷⁰-dependent according to its consensus sequence. Transcription of this promoter is also affected by the heat shock-specific sigma factor σ³²[48].