Figure 8

The RST domain of AtRCD1 is required for the TF interactions. The C-terminus of AtRCD1 was truncated to determine the minimum protein length capable of interacting with TFs. The dark gray horizontal bars above the AtRCD1 protein sequence denote the different constructs. Green background indicates interaction and gray background the lack of it. Yeast spots from each interaction test are depicted in the panel on the right. The ClustalW alignment of AtRCD1, AtSRO1 and AtSRO5 is included for comparison of the RST structure in different proteins. Highlighted AAs in the protein sequences are as in figure 7.