Figure 1

Knock-down of many genes previously associated with the TRAIL pathway reduces sensitivity to TRAIL-induced cell death. HeLa Cells were transfected with siRNAs targeting a selection of genes previously associated with TRAIL induced apoptosis. After 48 hrs the sensitivity of cells to treatment with 1 μg/ml TRAIL was measured (see Materials and Methods). Error bars represent 1 standard deviation (n = 3), * indicates significantly different from negative control (t-test on log-transformed data, Bonferroni correct p < 0.05).