The seed sequence ACTTGA protects cells from TRAIL-induced cell death. HeLa cells were transfected with siRNAs targeting Caspase-8 (siCasp8), Luciferase (siGL2) or siRNAs where the siGL2 sequence had been altered to contain the seed sequence ACTTGA (siGL2+seed) or the seed sequence ATCTGA (siGL2+mutseed). After 48 hrs the sensitivity to treatment with 0.5 μg/ml TRAIL was measured. Results are shown relative to cells not treated with TRAIL. Error bars represent 1 standard deviation (n = 3 biological replicates each with 3 technical replicates). Difference between siGL2-seed and siGL2-mutseed is significant (P < 2.5 × 10-3, t-test on log-transformed data).