Figure 4

miRNAs containing enriched seeds protect a several cell types from TRAIL induced cell death. Cells were transfected with miRNA mimics with same sequence as naturally occurring miRNAs that contain seed sequences enriched in high-scoring siRNAs, an miRNA mimic with a sequence occurring in no human miRNA, or an siRNA targeting Caspase-8 as a positive control. After 48 hrs the sensitivity to treatment with 0.5 μg/ml TRAIL was measured. Error bars represent 1 standard deviation (n = 3 bioligical replicates each with 3 technical replicates, * represents results significantly different from the negative control (P < 0.05, students' T-test on log-transformed data), ** are significantly different after Bonferroni correction.