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Table 1 Seeds and Confirmation status of the top 20 siRNAs

From: Systematic analysis of off-target effects in an RNAi screen reveals microRNAs affecting sensitivity to TRAIL-induced apoptosis

Transcript Symbol Score Hexamer Seed Heptamer Seed Status Enriched Seed
NM_003217 TEGT 3.51 CAAGGT TCAAGGT OTE +
NM_016368 ISYNA1 3.43 TGTCCA GTGTCCA OTE *
NM_001240 CCNT1 3.28 CAAGGT TCAAGGT OTE +
NM_002197 ACO1 3.11 ACTTGA CACTTGA Unconfirmed +
NM_003947 HAPIP 2.88 ACTTGA TACTTGA OTE +
NM_002337 LRPAP1 2.8 TCACAA ATCACAA OTE +
NM_005799 INADL 2.76 ACTTGA AACTTGA OTE +
NM_003554 OR1E2 2.72 TTGTAA TTTGTAA Unconfirmed -
NM_013345 GPR132 2.68 AGATCA GAGATCA Unconfirmed +
NM_004584 RAD9A 2.65 TAAGGA CTAAGGA OTE -
NM_003266 TLR4 2.64 AAAGTT CAAAGTT Unconfirmed -
NM_000674 ADORA1 2.63 TAATAA CTAATAA OTE +
NM_002382 MAX 2.57 AAGAAA TAAGAAA Hit -
NM_006986 MAGED1 2.56 ACTTGA AACTTGA False +ve +
NM_000875 IGF1R 2.55 CACTTA ACACTTA Hit -
NM_016953 PDE11A 2.54 GTAAGA GGTAAGA Hit -
NM_014379 KCNV1 2.54 TGTCCA GTGTCCA False +ve *
NM_080674 C20orf86 2.52 CGTTGT GCGTTGT False +ve -
NM_002467 MYC 2.51 TTACAC GTTACAC Hit +
XM_497793 LOC402037 2.51 GCATTA GGCATTA False +ve -
  1. Table shows the top 20 siRNAs from the screen, their status: Hit, False pos (did not repeat), Unconfirmed (that is this siRNA reproducibly affected caspase-3/7 activation levels, but we could not confirm the involvement of the gene targeted), or OTE (off-target effect), the hexamer and heptamer seed sequences (sequences that appear more than once are shown in bold), and whether the seed is one which either appears more than once, or is in the set of enriched seed sequences (see Table 2). * The seed sequence TGTCCA appears once in the OTE siRNA ISYNA1 and once in the false positive siRNA targeting KCNV1.