Figure 2

Differential gene expression in A. marginale -infected R. microplus male salivary glands. Real-time RT-PCR was done on uninfected and infected pooled salivary glands (two independent experiments). Genes up-regulated (white bars) and down-regulated (black bars) in infected salivary glands are shown. Bars represent average + SD mRNA ratios. The mRNA levels were normalized against tick β-actin using the comparative Ct method. The mRNA levels were compared between infected and uninfected tick salivary glands by Students's t test (*p ≤ 0.05). Gene IDs are described in additional file 2: Table S2.