Table 3 Real-time RT-PCR oligonucleotide primers and conditions.
EST | Upstream/downstream primer sequences (5'-3') | PCR annealing conditions |
|---|---|---|
7BstNI3 | AAACTGGGGAATCCAAAAGG GGGGTTTGGGATAGGGTTC | 55°C/30 s |
9PRB2 | AACGACCGCCCAAAAATAAC AATTTGTTCCGGTTTTGTTCC | 55°C/30 s |
22Hbp | GGAGGTTACGAACTATGGGC ATGAGTTGGCAGTGCCTTAG | 55°C/30 s |
28ImbpC | CGGTACCATGATGCACTTTG TGATGGCGTCCCTAGTTACC | 55°C/30 s |
36vATP | GAAGGCTTCGAACAGAGTCG CTCAATTCTGGTGGCCAAG | 55°C/30 s |
59Rib | CCAGCAAGCGAGATTGTGTA GCGTACTGTCTGAAGCAACG | 55°C/30 s |
88BstNI | GTTTGGGGGCCTTAAGAAAA TTTTTCCCAAAAGGTTCTCC | 55°C/30 s |
93 Meth | CTGAACTGAACGCATCATGG GCACAACATTTTGCAGATGG | 55°C/30 s |
94Will | TCATTGACGAAGAAGCGATC TACAAGTCGCCCTGACACC | 55°C/30 s |
100Silk | TGAACCAGAGGGACCAACTC GTCTTGGACTCGGCAGTAGC | 55°C/30 s |
104SrHb | CGAACCCGAATGGATTATG TTCAAACATGAAGCGACAGC | 55°C/30 s |
108Kunz | ATGGAACTGTTCGGTTTTGC ATCCGCCGTAAATGAAGTTC | 55°C/30 s |
120Ptse | GCGCGACCTCTTTGTTAAAC CGAATACGCACAGAAGGTGAC | 55°C/30 s |
128Pec | AGGCCCAATTCTGATCTTTC CAAAGCTCAAACGTGTGGTG | 55°C/30 s |
Subolesin (4D8) | GAGACCAGCCCCTGTTCA CTGTTCTGCGAGTTTGGTAGATAG | 54°C/30 s |
Beta-actin | GACATCAAGGAGAAGCT(TC)TGC CGTTGCCGATGGTGAT(GC) | 55°C/30 s |