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Figure 7 | BMC Genomics

Figure 7

From: Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana

Figure 7

Detection of epitope-tagged recombinant LRR-RLK proteins in Arabidopsis with Western hybridization. Full-length cDNAs of SERK1, SERK 2, SERK3, SERK4, SERK5 and BRI1 were recombined into pB35GWF and pK35GWG. FLAG-tagged fusion proteins, prepared by immunoprecipitation of total membrane protein, and total proteins from leaves of transgenic plants expressing GFP-tagged fusion proteins were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Proteins were transferred to nitrocellulose membrane and epitope-tagged proteins were detected using either (A) anti-FLAG or (B) anti-GFP antibody (diluted 1:3,000) followed by anti-mouse immunoglobulin G (IgG)-horseradish peroxidase (HRP) secondary antibody (diluted 1:10,000).

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BMC Genomics

ISSN: 1471-2164

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