Quantitative PCR and western blot of the relative abundance of neurofibromin in lymphoblastoid cell lines. A) Relative abundance of NF1 mRNA in Coriell-18 and ECACC-6 sets as detected by quantitative PCR. P values of respective t tests are shown above the bar plots. B) Western blot of neurofibromin in select lymphoblastoid cell lines (LCLs). TUBB - β-tubulin. C) Relative abundance of neurofibromin in select LCLs. NF1 abundance in sample E2-U was arbitrary set at 100%; NF1 abundance in the remaining LCLs is expressed as percentage of that in E2-U.