Quantitative PCR analysis of relative abundance of Nf1 mRNA in Nf1+/- mice. A) Plot of real-time amplification of Nf1 and Gapdh mRNAs in six wild-type and six Nf1+/- mice. Samples containing no input RNA are shown as "Negative control". B) Relative abundance of Nf1 mRNA from wild-type and Nf1+/- mice. P value of the t test is shown above the bar plot.