Skip to main content

Table 3 Quantitative PCR validation of microarray data for select human and mouse genes.

From: Evidence of perturbations of cell cycle and DNA repair pathways as a consequence of human and murine NF1-haploinsufficiency

Gene Reason for Validation Quantitative PCR P values
   Coriell-18 ECACC-6 Nf1 -Mouse-12
AUTS2 Overlap of Hs sets 0.0630 0.1790 N/A
C11orf75 Overlap of Hs sets 0.0127 0.2270 N/A
RAB31 Overlap of Hs sets 0.0168 0.0019 N/A
DUSP4 3-Way Overlap of Hs and Mm sets 0.0398 0.1910 N/A
AKAP2 Significant in Coriell-18 (I) 0.1170 0.3540 N/A
CNKSR3 Significant in Coriell-18 (I) 0.0026 0.1850 N/A
IFI6 Significant in Coriell-18 (I) 0.0013 0.5930 N/A
CHURC1 Significant in ECACC-6 0.7390 0.1450 N/A
GSG2 Significant in Nf1-Mm-12* 0.7210 0.1360 N/A
EGLN3 Significant in Coriell-18 (N) 0.0076 0.7630 N/A
MGST3 Significant in Coriell-18 (N) 0.0005 0.6991 N/A
POMC Significant in Coriell-18 (N) 0.0170 0.5582 N/A
Aytl2 Overlap of Hs-Mm sets N/A N/A 0.1900
Crip1 Overlap of Hs-Mm sets N/A N/A 0.5830
Pecr Overlap of Hs-Mm sets N/A N/A 0.5840
Zfp36l1 Overlap of Hs-Mm sets N/A N/A 0.5140
Dusp4 3-Way Overlap of Hs and Mm sets N/A N/A 0.7410
Cxcr3 Significant in Nf1-Mm -12* N/A N/A 0.0052
Gsg2 Significant in Nf1-Mm -12* N/A N/A 0.0262
Igtp Significant in Nf1-Mm -12* N/A N/A 0.0380
Inpp5k Significant in Nf1-Mm-12 N/A N/A 0.0720
Rnf43 Significant in Nf1-Mm -12* N/A N/A 0.0004
  1. Statistical significance of differential expression of genes was evaluated by t test. Genes with nominal P values < 0.05 were considered significant and are shown in boldface. "(I)" and "(N)" in "Significant in Coriell-18 (I)/(N)" indicate that sample set Coriell-18 was analyzed on the Illumina or NHGRI platform, respectively. "Hs" designates human (Coriell-18 and ECACC-6) datasets; "Mm" designates murine (Nf1-Mouse-12) dataset. * - nominal P value < 0.001. "N/A" - not applicable.