Figure 4

Gcn5 is associated with the transcribed region of the FLO8 gene and causes its repression during KCl stress. (A) Verification of FLO8 regulation and binding by Gcn5. The relative expression level of FLO8 gene in wild type and gcn5Δ cells under KCl stress conditions is shown (left panel) in relation to a control gene, YDL212W. Middle panel: tiling array data visualized by IGB showing relative enriched association of Gcn5-myc with FLO8 in immunoprecipiated material normalized against input material. The arrow shows the direction of transcription. A and B represent the location of the amplified binding region and control region used for semi quantitative PCR for ChIP, Right panel: semi quantitative PCR validation of Gcn5 association with FLO8 showing the level of Gcn5-myc associated chromatin in relation to the input for regions A and B as designated in the middle panel. (B) Diagram showing how deletion of Gcn5 causes indirect regulation of FLO8 target genes. FLO8Δ vs wild type microarray data are from ([32] GSE4654).