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Table 4 Results of BAC pools screening with molecular markers.

From: Physical mapping in highly heterozygous genomes: a physical contig map of the Pinot Noir grapevine cultivar

 

ESTs

SSRs

AFLPs

Markers not assigned to BAC clones*

36

25

13

   False positive or not fingerprinted BAC clones

16

7

8

   Not amplified in one of the six pools or over-represented in BAC clones

20

18

5

Markers assigned to BAC clones:

138

(4.9 clones*)

108

(3.6 clones*)

149

(2.0 clones*)

   Class I: identified two or more BACs assembled in a single contig

82

54

39

   Class II: identified only one BAC in a single contig

36

38

69

   Class III: identified singletons (unassembled BACs)

6

7

31

   Class IV: identified multiple BACs assembled into distinct contigs

14

9

10

Tested

174

133

162

  1. Following BAC screening, each single putatively positive BAC clone has been picked and tested to confirm the primary pool screening result.
  2. * average number of BAC clones identified for each marker type