The ancient mammalian KRAB zinc finger gene cluster on human chromosome 8q24.3 illustrates principles of C2H2 zinc finger evolution associated with unique expression profiles in human tissues

Table 3 Characteristics of the proximal gene promoters^a of the seven human 8q24.3 ZNF genes

Properties		ZNF7	ZNF16	ZNF34	ZNF250	ZNF251	ZNF252	ZNF517
Genomic location ^b	Start	146023180	146147578	145984033	146098150	145952275	146199589	145994565
	End	146023872	146146948	145983392	146097511	145951559	146198940	145995192
TSS ^c		528	501	505	501	501	501	501
		569	514		531	617	505	528
		590	531		535		544
		593			540		550
CpG island ^d		yes	yes	yes	yes	yes	yes	yes
Core promoter elements ^{e, f}	Position^g
INR	-2	no	no	no	no	no	no	no
TATA	-31	no	no	no	no	no	no	no
BRE	-37	no	no	no	no	no	no	no
INR-DPE	-2/+28	no	no	atypical	no	no	no	no
MTE	+18	atypical	atypical	yes	no	atypical	no	yes
XCPE	-8	no	yes	no	no	no	yes	no

^a Promoters determined by Genomatix Gene2Promoter (see Methods); sequences given in Additional file 11
^b Based on human genome hg18
^c TSS positions relative to first nucleotide in the defined promoter sequence (= 1)
^d As annotated in the UCSC genome browser http://genome.ucsc.edu, see Additional file 12
^e Elements predicted with Genomatix MatInspector (see Methods)
^f Scoring: yes = within 10 bp of usual position; atypical = not at typical position, but within 50 bp; no = absent or far (>50 bp) from typical position
^g Usual default starting position of the element with respect to TSS (= +1), [55]

ISSN: 1471-2164