TY - JOUR AU - Dragosits, Martin AU - Stadlmann, Johannes AU - Graf, Alexandra AU - Gasser, Brigitte AU - Maurer, Michael AU - Sauer, Michael AU - Kreil, David P. AU - Altmann, Friedrich AU - Mattanovich, Diethard PY - 2010 DA - 2010/03/26 TI - The response to unfolded protein is involved in osmotolerance of Pichia pastoris JO - BMC Genomics SP - 207 VL - 11 IS - 1 AB - The effect of osmolarity on cellular physiology has been subject of investigation in many different species. High osmolarity is of importance for biotechnological production processes, where high cell densities and product titers are aspired. Several studies indicated that increased osmolarity of the growth medium can have a beneficial effect on recombinant protein production in different host organisms. Thus, the effect of osmolarity on the cellular physiology of Pichia pastoris, a prominent host for recombinant protein production, was studied in carbon limited chemostat cultures at different osmolarities. Transcriptome and proteome analyses were applied to assess differences upon growth at different osmolarities in both, a wild type strain and an antibody fragment expressing strain. While our main intention was to analyze the effect of different osmolarities on P. pastoris in general, this was complemented by studying it in context with recombinant protein production. SN - 1471-2164 UR - https://doi.org/10.1186/1471-2164-11-207 DO - 10.1186/1471-2164-11-207 ID - Dragosits2010 ER -