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Table 1 Characteristics of P. pastoris X-33 grown in carbon limited chemostat cultures at different osmolarities.

From: The response to unfolded protein is involved in osmotolerance of Pichia pastoris

Clone Osmolarity [mOs kg-1] YDM [g L-1] Total protein supernatant [mg L-1] viability [%] forward scatter qP FabYDM-1h-1
wt 149 +/- 11,4 27.7 +/- 0.3 0.38 +/- 0.01 97.5 +/- 0.3 443 +/- 95 -
wt 865.7 +/- 3,2 27.3 +/- 0.2 0.36 +/- 0.02 98.8 +/- 0.2 258 +/- 15 -
wt 1351.3 +/- 2,0 26.0 +/- 0.3 0.38 +/- 0.00 98.1 +/- 0.2 297 +/- 4 -
Fab 135.2 +/- 3.3 27.8 +/- 0.2 0.47 +/- 0.04 97.2 +/- 0.4 355 +/- 60 0.039 +/- 0.004
Fab 857.3 +/- 8.5 26.8 +/- 0.4 0.44 +/- 0.05 97.9 +/- 0.6 225 +/- 30 0.042 +/- 0.002
Fab 1352 +/- 10,1 24.9 +/- 0.3 0.45 +/- 0.05 97.8 +/- 0.5 206 +/- 12 0.047 +/- 0.006
  1. wt represents the non-expressing wild type strain. Fab represents the recombinant protein producing strain. Osmolarity values represent actual measured values of the culture supernatant. +/- represents the standard error of the mean. - not applicable.