Table 1 Characteristics of P. pastoris X-33 grown in carbon limited chemostat cultures at different osmolarities.
From: The response to unfolded protein is involved in osmotolerance of Pichia pastoris
Clone | Osmolarity [mOs kg-1] | YDM [g L-1] | Total protein supernatant [mg L-1] | viability [%] | forward scatter | qP FabYDM-1h-1 |
|---|---|---|---|---|---|---|
wt | 149 +/- 11,4 | 27.7 +/- 0.3 | 0.38 +/- 0.01 | 97.5 +/- 0.3 | 443 +/- 95 | - |
wt | 865.7 +/- 3,2 | 27.3 +/- 0.2 | 0.36 +/- 0.02 | 98.8 +/- 0.2 | 258 +/- 15 | - |
wt | 1351.3 +/- 2,0 | 26.0 +/- 0.3 | 0.38 +/- 0.00 | 98.1 +/- 0.2 | 297 +/- 4 | - |
Fab | 135.2 +/- 3.3 | 27.8 +/- 0.2 | 0.47 +/- 0.04 | 97.2 +/- 0.4 | 355 +/- 60 | 0.039 +/- 0.004 |
Fab | 857.3 +/- 8.5 | 26.8 +/- 0.4 | 0.44 +/- 0.05 | 97.9 +/- 0.6 | 225 +/- 30 | 0.042 +/- 0.002 |
Fab | 1352 +/- 10,1 | 24.9 +/- 0.3 | 0.45 +/- 0.05 | 97.8 +/- 0.5 | 206 +/- 12 | 0.047 +/- 0.006 |