Table 2 Proteins that were affected by growth at different osmolarities in carbon limited chemostat cultures of P. pastoris X-33.
From: The response to unfolded protein is involved in osmotolerance of Pichia pastoris
| Â | Â | Â | Â | wt low/high | Fab low/high | ||
|---|---|---|---|---|---|---|---|
Spot no | Protein | description | MW/pI | Av ratio | 1-ANOVA | Av ratio | 1-ANOVA |
1 | Aco1 | aconitase | 84.5/5.93 | -2.25 | 3.70E-04 | 1.01 | 7.60E-04 |
2 | Aco1 | aconitase | 84.5/5.93 | -1.13 | 5.80E-02 | 1.54 | 4.40E-05 |
3 | Aco1 | aconitase | 84.5/5.93 | -1.23 | 8.40E-02 | 1.49 | 5.10E-04 |
4 | Aco1 | aconitase | 84.5/5.93 | 1.10 | 2.10E-01 | 1.51 | 2.30E-04 |
5 | Agx1 | alanine:glyoxylate aminotransferase | 31.0/6.36 | -1.79 | 1.00E-04 | -1.43 | 8.80E-03 |
6 | Aox1 | alcohol oxidase | 73.8/6.41 | 2.34 | 1.90E-02 | -1.36 | 1.80E-01 |
7 | Atp3 | F1F0 ATPase subunit | 31.6/7.74 | 3.28 | 3.20E-06 | 1.26 | 4.20E-01 |
8 | Cdc19 | pyruvate kinase | 49.6/6.24 | -1.81 | 3.40E-03 | -1.26 | 9.10E-03 |
9 | Cit1 | citrate synthase | 51.9/8.32 | 3.23 | 2.90E-04 | -1.04 | 1.30E-01 |
10 | Eft2 | Elongation Factor 2 | 93.6/6.29 | -1.93 | 3.40E-04 | 1.14 | 6.30E-02 |
11 | Erg10 | acetyl CoA acetyltransferase | 41.7/6.10 | -1.83 | 7.00E-05 | -1.20 | 5.30E-02 |
12 | Faa2 | long chain fatty acyl-CoA synth. | 25.4/6.73 | -1.65 | 3.40E-03 | 1.16 | 5.10E-01 |
13 | Fdh1 | formate dehydrogenase | 40.3/6.61 | 1.79 | 1.30E-03 | 1.35 | 3.20E-03 |
14 | Gdh1 | glutamate dehydrogenase | 49.3/5.67 | -2.38 | 3.40E-02 | -1.45 | 2.20E-04 |
15 | Gut1 | glycerol kinase | 68.2/5.33 | 1.31 | 5.90E-03 | 1.18 | 1.20E-03 |
16 | Hbn1 | nitroreductase (similar to bacterial) | 21.8/6.30 | 1.12 | 2.10E-01 | 1.36 | 3.00E-08 |
17 | Hsp60 | heat shock protein 60 | 60.2/5.08 | -1.83 | 1.20E-05 | -1.13 | 1.40E-04 |
18 | Icl1 | isocitrate lyase | 61.5/6.15 | 1.41 | 2.10E-03 | 1.56 | 1.90E-05 |
19 | Ino1 | inositol-1-P synthase | 58.4/5.26 | -2.61 | 1.30E-05 | -1.88 | 1.30E-04 |
20 | Ino1 | inositol-1-P synthase | 58.4/5.26 | -1.78 | 1.80E-05 | -1.11 | 1.60E-04 |
21 | Ino1 | inositol-1-P synthase | 58.4/5.26 | -1.06 | 3.60E-01 | 1.41 | 9.00E-04 |
22 | Kar2 | BiP | 74.2/4.79 | -2.95 | 4.70E-05 | -1.05 | 5.90E-01 |
23 | Pab1 | poly A binding protein | 68.6/5.07 | -1.77 | 5.00E-04 | -1.03 | 3.70E-05 |
24 | Pdi1 | protein disulfide isomerase | 57.8/4.63 | -1.66 | 2.10E-05 | 1.08 | 1.00E-02 |
25 | Pgi1 | phosphoglucose isomerase | 61.9/5.83 | 1.12 | 1.00E-01 | 1.07 | 7.00E-04 |
26 | Pgk1 | phosphoglycerate kinase | 44.1/7.77 | -1.97 | 3.30E-05 | -1.03 | 2.40E-02 |
27 | Pil1 | Primary component of eisosomes | 35.3/5.03 | 1.35 | 2.60E-02 | 1.12 | 6.20E-03 |
28 | Rib3 | DHBP synthase/riboflavin | 22.9/5.09 | -1.84 | 7.80E-06 | -1.16 | 7.60E-02 |
29 | Sor2 | similar to sorbitol dehydrogenase | 38.6/5.76 | 1.34 | 3.50E-02 | 1.24 | 2.50E-03 |
30 | Ssa4 | heat shock protein | 70.3/5.12 | -1.20 | 4.90E-02 | 1.15 | 1.10E-05 |
31 | Ssb1 | heat shock protein | 66.5/5.12 | -1.02 | 8.70E-01 | 1.17 | 1.70E-03 |
32 | Ssb1 | heat shock protein | 66.5/5.12 | 1.58 | 1.20E-03 | 1.03 | 1.50E-05 |
33 | Ssc1 | mitochondrial matrix ATPase | 69.7/5.71 | -4.7 | 3.80E-03 | -1.20 | 5.40E-05 |
34 | Sse1 | hsp70 family ATPase | 78.7/5.11 | -2.79 | 5.80E-05 | -1.14 | 3.70E-04 |
35 | Ssz1 | hsp70 family ATPase | 57.9/4.83 | -1.81 | 8.00E-06 | -1.21 | 4.30E-05 |
36 | Tfs1 | carboxypeptidase Y inhibitor | 24.2/4.92 | -2.00 | 3.90E-04 | -1.39 | 2.00E-02 |
37 | Ymr090W | unknown function | 25.1/6.91 | -1.41 | 3.40E-05 | -1.23 | 1.30E-04 |