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Table 2 Proteins that were affected by growth at different osmolarities in carbon limited chemostat cultures of P. pastoris X-33.

From: The response to unfolded protein is involved in osmotolerance of Pichia pastoris

     wt low/high Fab low/high
Spot no Protein description MW/pI Av ratio 1-ANOVA Av ratio 1-ANOVA
1 Aco1 aconitase 84.5/5.93 -2.25 3.70E-04 1.01 7.60E-04
2 Aco1 aconitase 84.5/5.93 -1.13 5.80E-02 1.54 4.40E-05
3 Aco1 aconitase 84.5/5.93 -1.23 8.40E-02 1.49 5.10E-04
4 Aco1 aconitase 84.5/5.93 1.10 2.10E-01 1.51 2.30E-04
5 Agx1 alanine:glyoxylate aminotransferase 31.0/6.36 -1.79 1.00E-04 -1.43 8.80E-03
6 Aox1 alcohol oxidase 73.8/6.41 2.34 1.90E-02 -1.36 1.80E-01
7 Atp3 F1F0 ATPase subunit 31.6/7.74 3.28 3.20E-06 1.26 4.20E-01
8 Cdc19 pyruvate kinase 49.6/6.24 -1.81 3.40E-03 -1.26 9.10E-03
9 Cit1 citrate synthase 51.9/8.32 3.23 2.90E-04 -1.04 1.30E-01
10 Eft2 Elongation Factor 2 93.6/6.29 -1.93 3.40E-04 1.14 6.30E-02
11 Erg10 acetyl CoA acetyltransferase 41.7/6.10 -1.83 7.00E-05 -1.20 5.30E-02
12 Faa2 long chain fatty acyl-CoA synth. 25.4/6.73 -1.65 3.40E-03 1.16 5.10E-01
13 Fdh1 formate dehydrogenase 40.3/6.61 1.79 1.30E-03 1.35 3.20E-03
14 Gdh1 glutamate dehydrogenase 49.3/5.67 -2.38 3.40E-02 -1.45 2.20E-04
15 Gut1 glycerol kinase 68.2/5.33 1.31 5.90E-03 1.18 1.20E-03
16 Hbn1 nitroreductase (similar to bacterial) 21.8/6.30 1.12 2.10E-01 1.36 3.00E-08
17 Hsp60 heat shock protein 60 60.2/5.08 -1.83 1.20E-05 -1.13 1.40E-04
18 Icl1 isocitrate lyase 61.5/6.15 1.41 2.10E-03 1.56 1.90E-05
19 Ino1 inositol-1-P synthase 58.4/5.26 -2.61 1.30E-05 -1.88 1.30E-04
20 Ino1 inositol-1-P synthase 58.4/5.26 -1.78 1.80E-05 -1.11 1.60E-04
21 Ino1 inositol-1-P synthase 58.4/5.26 -1.06 3.60E-01 1.41 9.00E-04
22 Kar2 BiP 74.2/4.79 -2.95 4.70E-05 -1.05 5.90E-01
23 Pab1 poly A binding protein 68.6/5.07 -1.77 5.00E-04 -1.03 3.70E-05
24 Pdi1 protein disulfide isomerase 57.8/4.63 -1.66 2.10E-05 1.08 1.00E-02
25 Pgi1 phosphoglucose isomerase 61.9/5.83 1.12 1.00E-01 1.07 7.00E-04
26 Pgk1 phosphoglycerate kinase 44.1/7.77 -1.97 3.30E-05 -1.03 2.40E-02
27 Pil1 Primary component of eisosomes 35.3/5.03 1.35 2.60E-02 1.12 6.20E-03
28 Rib3 DHBP synthase/riboflavin 22.9/5.09 -1.84 7.80E-06 -1.16 7.60E-02
29 Sor2 similar to sorbitol dehydrogenase 38.6/5.76 1.34 3.50E-02 1.24 2.50E-03
30 Ssa4 heat shock protein 70.3/5.12 -1.20 4.90E-02 1.15 1.10E-05
31 Ssb1 heat shock protein 66.5/5.12 -1.02 8.70E-01 1.17 1.70E-03
32 Ssb1 heat shock protein 66.5/5.12 1.58 1.20E-03 1.03 1.50E-05
33 Ssc1 mitochondrial matrix ATPase 69.7/5.71 -4.7 3.80E-03 -1.20 5.40E-05
34 Sse1 hsp70 family ATPase 78.7/5.11 -2.79 5.80E-05 -1.14 3.70E-04
35 Ssz1 hsp70 family ATPase 57.9/4.83 -1.81 8.00E-06 -1.21 4.30E-05
36 Tfs1 carboxypeptidase Y inhibitor 24.2/4.92 -2.00 3.90E-04 -1.39 2.00E-02
37 Ymr090W unknown function 25.1/6.91 -1.41 3.40E-05 -1.23 1.30E-04
  1. Analysis was performed by 2D-DIGE and subsequent LC ESI-MS/MS identification. Protein standard name (according to the SGD, http://www.yeastgenome.org), protein functional description, theoretical molecular weight (MW) and theoretical isoelectric point (pI), average expression values and 1-ANOVA values are shown. Significant ANOVA values are indicated in bold letters. The average protein abundance fold change of the comparison of low and high osmolarity setpoints (Av ratio) are shown. Additional protein fold change data are available through Additional file 3.