Transcript detection around CW- orf307 locus by northern blot analysis. (A) C-terminus of CW-orf307 and the downstream sequences were probed. (B) Northern blot analysis of mitochondrial RNA extracted from calli using probes 1 to 3. atp6 was used as the internal control. (C) Northern blot using probe 1 to detect CW-orf307 transcript using the total RNA extracted from anthers at the tricellular pollen stage. R, CWR (The restorer line carrying Rf17 and CW cytoplasm); C, CW-CMS line; T, Taichung 65.