Table 4 Primer list
From: Testes and brain gene expression in precocious male and adult maturing Atlantic salmon (Salmo salar)
Gene | Accession | Primer species | Primer | Primer sequences |
|---|---|---|---|---|
Anti Mullerian Hormone | AY722411 | S.salar | AMHF | ACAAGTGTTCGATCCAGACGTGAC |
| Â | Â | Â | AMHR | CACTCAGTCTGCCTTGGTGTGG |
Elongation factor 1 alpha | AF321836 | S.salar | EF1aF | TAAGGGCAACAGCAGTGGCAGTG |
| Â | Â | Â | EF1aR | CGCATTTGTAGATCAGATGGCCG |
Beta-globin | BT050121 | S.salar | BGF | CCCATGGCTGCGACAACCACTTTC |
| Â | Â | Â | BGR | CAACACACTCTTCGTCGACCCTGAC |
Beta-2-microglobulin | NM_001123699 | S.salar | RTB2F | GTACTTGTGCTCATTTACAGCGCGG |
| Â | Â | Â | RTB2R | GCCACTCACGTGACAGATCAGGG |
1Type 1 collagen | BE518482 | S.salar | T1C1F | GAGGCAATGACCGATGGCTTC |
| Â | Â | Â | T1C1R | GCGATGCTGTTCTTGCAGTGG |
Cathepsin B | EG934102 | S.salar | CatBF | TGTGAGACTGGATACACACCTGGCTAC |
| Â | Â | Â | CatBR | GCTCCTTCCACAGGTCCGTTCTTC |
Guanine nucleotide binding protein | BT048931 | S.salar | GNBPF | GTCGCCAAAATGACCGAGCAG |
| Â | Â | Â | 2GNBPR | GTCTCATCACGGGTCAACTTCCAC |
Lipoprotein lipase | EG868885 | S.salar | LIPF | CGGCCCGACCTTTGAGTTTG |
| Â | Â | Â | LIPR | TTGGGGTAGATGTCCACGTGGC |
Apolipoprotein E | NM_131098 | D. rerio | ApoEF | TCTCTTGTGGTATTCTTTGCCCTGG |
| Â | Â | Â | ApoER | GTTCAGACACATACTGCCAGAAACGG |
Calponin 3 | BC053309 | D. rerio | Cal3F | GCAACTGTGATTTTACTGCAACTTTAGGAC |
| Â | Â | Â | Cal3R | CAATCTTGTTTTTCACCTCCGCG |
Isotocin 1 | BT049938 | S.salar | SsIT1F | AAAGCCTCAACCTCAACACATGGC |
| Â | Â | Â | SsIT1R | CTGGATGGGAAAGCTAGTGCTGA |
Isotocin 2 | CA063528 | S.salar | SsIT2F | TCAGTAAATGGGTGGGTGAAATAGGTG |
| Â | Â | Â | SsIT2R | AGCTGCAAGTGACGCCAGGGT |
02A07 | FD425665 | S.salar | 02A07F | ACCATGTTGACAGCCAGTTTGCG |
| Â | Â | Â | 02A07R | TTCCGCACTCTCAAGCTCACCAC |
04H01 | GT145175 | S.salar | 04H01F | ACTGTAGTAGTCAGCTTGTGAAGGATGC |
| Â | Â | Â | 04H01R | GTAGTTCCATAGACAGAGAATGGATGCTC |
22D02 | FD425557 | S.salar | 22D02F | CAGCTGCTAATACAGGGTTATTGTTTTG |
| Â | Â | Â | 22D02R | CACGTTTATGACAACTGACACGTG |
3MCH1 | M25754 | O.tshawytscha | MCH1F | AAGAGGCCGACCAGGACCTGA |
| Â | Â | Â | MCH1R | ACTCAAGATGAGGCAGGACAAGATGC |
MCH2 | M25755 | O.tshawytscha | MCH2F | TCCCCATCGGAAAGACGGAG |
| Â | Â | Â | MCH2R | TTCAGGTTCATCCACAGGCCA |
Ubiquitin | BT060103 | S.salar | UBQF | CCACAAAAAGCACCAAGCCAAC |
| Â | Â | Â | UBQR | AGCTGGCCCAGAAGTACAACTGTG |
Beta-actin | NM_001123525 | S.salar | ACTF | ATGGAAGATGAAATCGCCG |
| Â | Â | Â | ACTR | CCCTCTTGCTCTGAGCCTCG |
Succinate de-hydrogenase | NM_001141694 | S.salar | SUCDEF | GAGGGAAAGGAACAATACCATCAGACTG |
| Â | Â | Â | SUCDER | GACAGCAGGTCCCAGGTACTTGTCTC |