Figure 2
From: Acetylation of p65 at lysine 314 is important for late NF-κB-dependent gene expression
Confirmation of gene expression profiles by qRT-PCR. Gene induction of Mmp10 (A), Mmp13 (B), Cfb (C) and Mpa2l (D) in a TNFα-dependent manner, as measured by real-time RT-PCR, from the following complemented cell lines: wild type (green), KTR (red), K314/315R (blue) and pTV (grey). Samples were normalized to Rps6 and CanX expression levels, and expressed as fold increase relative to wild type unstimulated. Two biological replicates were included. Shown are the means ± SD of three independent runs.