Figure 3

Comparison of real-time PCR and microarray data of ten randomly selected genes. Real-time PCR analysis was performed on cDNA derived from five biological samples of B. pahangi and B. malayi microfilariae with similar age distribution. (A) The ratio of Ct values obtained following real-time PCR analysis using primers flanking three probes shown to have similar signal intensities in the microarray data. Probe bm.02018, which represented Pub locus Bm1_34045, was assigned as the endogenous control gene. (B) Comparison of log-transformed microarray data (three biological samples) and real-time PCR data (five biological samples) of the ten representative genes showing good agreement of expression values obtained from both methodologies. Biological samples used for real-time PCR analysis were independent of those used for microarray analysis.