Transcriptomic profiling of bovine IVF embryos revealed candidate genes and pathways involved in early embryonic development

Table 3 Primer sequences in real- time RT-PCR reactions and products' sizes

Gene	Primer	Sequence (5' - 3')	Amplicon (bp)
GAPDH	Forward	TGCCCAGAATATCATCCC	134
	Reverse	AGGTCAGATCCACAACAG
PHLDA2	Forward	CCTAAGTCCCACGGCGAATC	109
	Reverse	CTATATCCTTGCCCTGGTCAGC
FERMT2	Forward	GATTAGGATGGACGCCAGCAC	128
	Reverse	AGGACAACCGTACTTCATCTGC
SHISA2	Forward	GCGGCTGCGACAACGATC	130
	Reverse	ATGAAGGCGACAAACACTGACC
RP2	Forward	AAGCACCTGACTTCCTTCCTC	119
	Reverse	CTTGGTCCCTTTGAATGTCTCG
TGFBR3	Forward	TCGCTGGATGCCTCAATG	140
	Reverse	ATCTGTGGAGTAATTGGAATCG
MCF2L	Forward	TGAGCCTGGAGGGATACG	110
	Reverse	GCCATCGTTGTCCTCAGG
SLC11A2	Forward	TGCAGTGGTCAGCGTAGC	111
	Reverse	TTAGAGATGCTTACCGTGTGC
SERPINC1	Forward	AAGTCCAGGCTCCCAGGTATTG	142
	Reverse	GCGAACGACCAGCGATGC
FDFT1	Forward	GGTCACCCTGATGATGGATGC	139
	Reverse	CCTGATGGTGGAGATGATCTGC
ACTB	Forward	AGGCCAACCGTGAGAAGATGAC	100
	Reverse	CCAGAGGCATACAGGGACAGC
RPLP0	Forward	GACAATGGCAGCATCTAC	198
	Reverse	GAAGGTGTAATCAGTCTC

ISSN: 1471-2164