Visualization and validation of copy number variation region (chr15:1836732-2039483) by visual examination and quantitative PCR. (A) Visualization of identified individual copy number variations in UCSC Genome Browser. The black bars indicate copy number variation of each sample. (B) Determination of copy number by quantitative PCR around third marker (Hapmap24310-BTA-162764). (C) Visual examination by consecutive genoplot images of markers. The first marker shows a monomorphic pattern having 2× (color: blue), 1× (color: cyan), and 0× (color: black). The samples having a deletion (copy number: 1×; color:cyan) were consecutively displayed in deletion position to the fourth marker.