Protein evolution in genes with different subcellular localization. Boxplots of nonsynonymous substitution rates dN of coding sequences averaged for all 36 strains. Data were grouped by subcellular localization as annotated in the Pseudomonas genome database . 87 genes showing a very low read depth (≤ 25% of the average read depth) were neglected in this analysis. Boxplots indicate 0.25 and 0.75 quantiles and the median (red line) with notches giving an estimate for the variance of the median. The green dashed line indicates the median dN for all genes. Values with dN above 20 × 10-3 are not shown.