Table 1 Correlation of transcripts and proteins of the polyamine biosynthetic pathway following PfSpdSyn inhibition.
| Â | Fold change (relative to control) | |||||
|---|---|---|---|---|---|---|
Annotation PlasmoDB ID | Hours post invasion | Oligos representing gene | Oligonucleotide array | RT-qPCR | Proteomics | |
| Â | 18 | 1 | -2.304 | -3.472 | nd | |
Adenosine deaminase, putative PF10_0289 | 25 | 1 | -2.907 | -5.495 | nd | |
| Â | 30 | 1 | -2.778 | -3.413 | -3.030 | |
| Â | 18 | 1 | -3.559 | -4.651 | nd | |
Purine nucleoside phosphorylase/uridine phosphorylase, putative PFE0660c | 25 | 1 | -3.731 | -5.882 | -2.222 | |
| Â | 30 | 1 | -3.367 | -4.348 | nd | |
| Â | 18 | 1 | -1.961 | -3.115 | nd | |
Phosphoethanolamine N-methyltransferase, putative MAL13P1.214 | 25 | 1 | -3.401 | -5.495 | nd | |
| Â | 30 | 1 | -3.774 | -4.049 | nd | |
| Â | 18 | 2 | -1.157 | 1.123 | -1.185 | nd |
Spermidine synthase PF11_0301 | 25 | 2 | -1.038 | 1.300 | -1.289 | nd |
| Â | 30 | 2 | -1.035 | 1.320 | +1.087 | nd |
| Â | 18 | 1 | +1.324 | -1.362 | nd | |
Lysine decarboxylase, putative PFD0285c | 25 | 1 | +2.426 | +1.106 | nd | |
| Â | 30 | 1 | +1.954 | +1.591 | nd | |
| Â | 18 | 1 | -1.300 | nd | -3.030 | |
Ornithine aminotransferase, PFF0435w | 25 | 1 | +1.044 | nd | nd | |
| Â | 30 | 1 | +1.182 | nd | nd | |
| Â | 18 | 1 | -1.597 | nd | -2.857 | |
S-adenosyl methionine synthethase, PFI1090 | 25 | 1 | -1.631 | nd | -2.222 | |
| Â | 30 | 1 | -1.761 | nd | nd | |
| Â | 18 | 1 | 1.347 | Â | nd | |
Eukaryotic translation initiation factor 3 subunit 10, putative (endogenous control) PFL0625c | 25 | 1 | 1.210 | endogenous control | nd | |
| Â | 30 | 1 | 1.221 | Â | nd | |