Figure 5
From: Thioredoxin and glutathione systems differ in parasitic and free-living platyhelminths
Expression of S. mansoni variants detected by RT-PCR. A. Schematic representation of the three mRNA variants of S. mansoni TGR, the variant 1 and variant 2 specific primers (F1 and F2) and the primer corresponding to the 5'-end of the cytosolic variant (F3) used in RT-PCRs in combination with a reverse primer derived from exon 3 sequence. B. Electrophoresis of PCR products from S. mansoni cercariae: a band of the expected size was observed in the PCRs with F1, F2 and F3 primers and the reverse primer (lanes 2 to 4, respectively). MWM lane corresponds to 100 bp plus ladder (Fermentas).