Figure 9
Genes related to flagellum synthesis and regulation. (A) Synteny and hybridization results between XAC e XauB/XauC. In the upper left diagram, F1, F2 and F3 denote gene regions related to flagellar functions, whereas gene groups XACSR9 and Y appear to contain unrelated genes. Dashed boxes denote regions apparently absent from the XauB and XauC genomes. Horizontal green bars represent hybridization signals for probes marked with Cy3 and which are XAC-specific; yellow horizontal bars represent regions with positive hybridization results. The zoomed-in region XACSR9 shows that it contains four transposases (in blue), twelve hypothetical genes (in gray), and one gene with assigned function (in yellow). That gene (XAC1927) codes for a Fe-S-oxidoreductase that contains a RADICAL SAM domain. XAC mutants in this particular region (14H02 and 25D11 [31]) presented decreased virulence phenotypes. (B) Microscopy and motility test of each organism. These results validate flagellum presence and functionality in two different culture media (DYGS and TSA). (C) Flaggellum gene organization in gene clusters F1, F2 and F3, as given in panel A. The color coding is the same for panel D. (D) Representation of the flagellum proteins using the same color coding as in panel C.