Sequence alignment of the esp PAI in E. faecium E1162, E1679 and U0317. The red-colored bands represent matching regions between the esp PAIs. A small blue band represents the inversion of a transposase fragment between E. faecium E1162 and E1679. Arrows indicate CDS and direction of transcription. The yellow arrows indicate the fragment of the esp PAI, that is identical to a fragment of the PAI of E. faecalis MMH594 . The green arrows indicate the CDS that are homologous to EfaB5. The purple arrows in E1679 indicate the CDS predicted to encode the pathway of inositol metabolism. Orange arrows indicate transposases and integrases. White arrows indicate flanking genes that are not part of the esp PAI. The positions of the rpsI, tuf and esp genes are indicated.