Whole genome assembly of a natto production strain Bacillus subtilis natto from very short read data

BMC Genomics

Table 1 Summary of sequence reads, coverage, and quality score.

Strain	Genomic DNA	Total reads	Mapped reads	Coverage	Quality score 40
	(ng/ *μ l)*	(million)	(%)	(fold)	(%)
BEST 195	207	15.3	79.1	131	86.3
Marburg 168	140	13.2	92.2	111	99.7

Genomic DNA was extracted from B. subtilis natto BEST195 isolated from Miyagino-based natto, and B. subtilis Marburg 168 (1A1). The short paired-end reads were generated by the Illumina genome analyzer GA2. The generated paired-end reads were mapped to the Marburg 168 reference genome using MAQ software. At each base, a quality score was statistically calculated based on the reads using MAQ. The quality scores describe the confidence that the base is correctly called.

ISSN: 1471-2164