Whole genome assembly of a natto production strain Bacillus subtilis natto from very short read data

BMC Genomics

Table 2 Summary of scaffolds produced by Velvet and their sorting.

Strain	Number of total scaffolds	Average length	Total length	N50 scaffold size (bp)	Number of scaffolds longer	Number of aligned scaffolds
		(bp)	(kbp)		than 1 kbp
BEST 195	390	6,693	4,178	72,513	117	84
Marburg 168	205	11,319	4,222	489,616	45	45

All short paired-end reads generated by the Illumina GA2 were input into the de novo assembly software Velvet and a set of scaffolds produced. The scaffolds greater than 1 kbp were aligned to the Marburg 168 reference genome. N50 indicates the scaffold length such that 50% of the de novo assembled sequences lies in scaffolds of this size or larger.

ISSN: 1471-2164