sRNA candidates validated by Northern hybridizations and 5'-RACE. Sequence coverage profile: blue and light grey color denote transcript coverages derived from sample 1 and 2, respectively. Dark grey colored areas represent an overlap of coverages from both samples. y- and x-axis represent coverage and sequence, respectively. Sequence code: blue, A; yellow, C; orange, G; green, U. Grey arrows represent genes flanking or overlapping sRNA genes. Black arrows represent the sRNAs. MFE: minimum free energy within the shape class. Validated by Northern hybridizations: trans-encoded sRNAs SmelB064 (a) and SmelC775 (b). Validated by 5'-RACE: trans-encoded sRNAs SmelB169 (c), SmelA075 (d), SmelB032 (e), SmelA060 (two copies in the genome, second copy SmelA072) (f). Lanes: 1, TY (control for cold shock); 2, cold shock; 3, TY (control for heat shock); 4, heat shock; 5, GMX (control for salt chock); 6, salt shock.