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Figure 2 | BMC Genomics

Figure 2

From: High-content siRNA screening of the kinome identifies kinases involved in Alzheimer's disease-related tau hyperphosphorylation

Figure 2

Quantitative reduction of 12E8 tau with siRNA against MARK2. Shown in A is detection of pS262 tau and total tau levels following transfection with MARK2 siRNA. Our quantitative assay detects significant reduction of pS262 tau using siRNA directed at the microtubule affinity regulating kinase 2 (MARK2) gene (red panels; p = 0.029 for three replicates). MARK2 siRNA has no effect on the overall levels of total tau protein (green panels), demonstrating that this assay can specifically detect reductions in pS262 tau. Representative images are shown. Percent control numbers represent the average of three independent assays. NS refers to the non-silencing siRNA control. In B, qRT-PCR results confirmed >95% knockdown of MARK2 mRNA relative to non-silencing siRNA control (NS).

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