Figure 3

DYRK1A is required for efficient phosphorylation of 12E8 tau. Shown in A are results of siRNA screening for DYRK1A siRNA. 12E8 tau is shown in red and total tau is shown in green (see Experimental Methods). For B, H4 cells overexpressing four repeat tau (4R0N) were transfected with siRNA targeting the DYRK1A transcript. Silencing of DYRK1A was confirmed with anti-DYRK1A antibody (top panel). Percent control values represent the average of three independent siRNA transfections and westerns. In A and B, NS refers to the nonsilencing control.