Figure 3
Tissue-specific gene expression. Panel A shows a selection of transcripts identified by ANOVA analysis followed by Pavlidis Template Matching analysis. The genes are clustered according to their expression profile for the analyzed tissues (Pearson correlation, average linkage clustering). The expression value is indicated by a sliding scale, going from black, indicating no expression values, through blue and green to red for the most highly expressed genes. Panel B show the tissue specific expression verification using qRT-PCR of two of the genes showed in panel A: the ferret albumin gene, Alb, and the ferret pulmonary surfactant-associated protein C, Sftpc. The graphs depict the log2-transformed curves of the relative fluorescence against cycle number during the qRT-PCR amplification. The analysis was done using RNA extracted from liver, lung, blood and brain, using the Atf4 housekeeping gene as reference. The C(t) threshold line (indicated by a red solid line) is set automatically using the noise band as cut off.