Figure 4
Panel A: Hierarchical clustering analysis showing a set of possible housekeeping genes. These genes were derived from the list of human housekeeping genes generated by Eisenberg and Levanon et al [24], and further selected using ANOVA analysis. The red and the blue bar indicate the Nono and the Atf4, shown in greater detail in Panel B. The green bar indicates the expression profile of some of the most commonly used housekeeping genes. Panel B: Normalized, scaled and log2-transformed probe intensities from Nono and Atf4 across the tissues. On average, there was a 3.57 fold difference between these two genes. Panel C shows the independent confirmation of the expression differences between the Nono and Atf4 in the liver. Quantitative RT-PCR determined Atf4 to have a 2.99 times higher expression than Nono, which resembled the array data well.