UGA serves as a codon for selenocysteine and a polyadenylation signal in addition to termination in S. barkhanus and S. salmonicida. (A) Phylogenetic tree of putative S. barkhanus selenophosphate synthetase with homologs representing the diversity of the protein family. Eukaryotes, Archaea and Bacteria are shown in red, blue and black, respectively. Maximum likelihood bootstrap values and bayesian posterior probabilities are shown above and below branches, respectively. GenBank identification numbers (gi) for each sequence are shown in parentheses. (B) Alignment of a putative S. barkhanus selenoprotein W1 (contig253) with homologs from diplomonads (S. salmonicida - gi:120476942, G. lamblia - gi:253741748, and S. vortens ), and a selection of other eukaryotes (Homo sapiens - gi:4506887, Danio rerio - gi:29648542, Chlamydomonas reinhardtii - gi:159471514, Bigelowiella natans - gi:47028259). (C) Sequence logo  around the termination codon of 134 S. barkhanus cDNA sequences.