Large genomic differences between the morphologically indistinguishable diplomonads Spironucleus barkhanus and Spironucleus salmonicida

Table 1 PCR amplifications of regions with SNPs observed in the EST data.

Gene	species ^a	PCR		#SNP			#alleles PCR		#clones major and (minor) alleles
		length	#clones	PCR	EST	shared	total	major ^b
Enolase	SB	566	14	4	4	4	4	3	6,4,3 (1)
Enolase	SS	566	10	0	0	0	1	1	10
Ribosomal protein S2	SB	496	10	0	0	0	1	1	10
Ribosomal protein S2	SS	499	10	0	0	0	1	1	10
Glutamate dehydrogenase	SB	549	13	6	2	1	2	2	9,4
Glutamate dehydrogenase	SS	549	13	10	N/A^c	N/A^c	3	2	8,4 (1)
HSP70	SB	514	67^d	5	5	4	6	5	54,5,4,2,1 (1)
HSP70	SS	514	10	0	N/A^c	N/A^c	1	1	10
Pyruvate kinase	SB	466	68^d	13	9	9	11	3	43,8,6 (2,2,2,1,1,1,1,1)
Pyruvate kinase	SS	466	10	0	0	0	1	1	10

^a) SB: Spironucleus barkhanus, SS: Spironucleus salmonicida
^b) Major alleles are the minimal set of alleles observed in the PCR experiments to explain all SNPs present in the data.
^c) No S. salmonicida ESTs were available for these two genes.
^d) From two independent PCR reactions.

ISSN: 1471-2164