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Table 1 PCR amplifications of regions with SNPs observed in the EST data.

From: Large genomic differences between the morphologically indistinguishable diplomonads Spironucleus barkhanus and Spironucleus salmonicida

Gene species a PCR #SNP #alleles PCR #clones major and (minor) alleles
   length #clones PCR EST shared total major b  
Enolase SB 566 14 4 4 4 4 3 6,4,3 (1)
Enolase SS 566 10 0 0 0 1 1 10
Ribosomal protein S2 SB 496 10 0 0 0 1 1 10
Ribosomal protein S2 SS 499 10 0 0 0 1 1 10
Glutamate dehydrogenase SB 549 13 6 2 1 2 2 9,4
Glutamate dehydrogenase SS 549 13 10 N/Ac N/Ac 3 2 8,4 (1)
HSP70 SB 514 67d 5 5 4 6 5 54,5,4,2,1 (1)
HSP70 SS 514 10 0 N/Ac N/Ac 1 1 10
Pyruvate kinase SB 466 68d 13 9 9 11 3 43,8,6 (2,2,2,1,1,1,1,1)
Pyruvate kinase SS 466 10 0 0 0 1 1 10
  1. a) SB: Spironucleus barkhanus, SS: Spironucleus salmonicida
  2. b) Major alleles are the minimal set of alleles observed in the PCR experiments to explain all SNPs present in the data.
  3. c) No S. salmonicida ESTs were available for these two genes.
  4. d) From two independent PCR reactions.