Figure 2

Images of RT-PCR for testing gene expression of 9 annotated sugarcane genes inferred from sorghum ESTs and predicted genes. Seven samples were used for RT-PCR amplification, and they are 1. S. officinarum LA Purple mature leaf cDNA (LAP-L); 2. LA Purple leaf roll cDNA (LAP-LR); 3. An F1 individual 95-4655 mature leaf cDNA (95-L); 4. 95-4655-leaf roll cDNA(95-LR); 5. BAC DNA as positive control (BAC); 6. Total RNA without DNase treatment from both leaf and leaf roll of LAP; 7. Total RNA with DNase treatment from both leaf and leaf roll of LAP. Genes 1, 2, 3, 5, 8, and 9 are expressed with Gene 5 being leaf roll specific and Gene 8 mature leaf specific. No gene expression were detected in Genes 4,6 and 7.