Figure 6

nfs genes are upregulated late during starvation induced development and expressed only within sporulating cells. A. The starvation-induced developmental program was induced in submerged culture for strains expressing mCherry fused after the promoter from pilA (P _pilA ::mCherry; strain PH1221), the 500 bp upstream of nfsA (P _nfsA ::mCherry; strain PH1220) or the vector backbone (vector; strain PH1222) in the DK1622 background. At the indicated times, mCherry fluorescence was recorded in a plate reader. B. Constructs expressing mCherry fused after the promoter from pilA (P _pilA ::mCherry; strain PH1221), the 500 bp upstream of nfsA (P _nfsA ::mCherry; strain PH1220) or the vector backbone (vector; strain PH1222) in the DK1622 background were induced to develop under submerged culture for 36 hours. Peripheral rods and fruiting body spores were separated and examined by fluorescence (left images) or DIC (right images) microscopy.