Comparison of the microarray and in situ hybridization results. The log2 of the red/green ratio for the microarray analysis is plotted on the left for the specified probe while the volumetric density (Vvi) for the in situ hybridization (ISH) analysis is plotted on the right for the specified probe. For the microarray analysis, the data for each time point of treatment (refer to Figure 2) are plotted with a line representing the mean of the data. The bigger, light-colored squares represent the time points that correlate with the ISH data. For the ISH, the Vvi for 15 fields at 400× magnification for the single time point is plotted with a line representing the mean of the data. A student's t-test was performed on the ISH data, and all p-Values were < 0.0001, indicating that there is a significant difference between uninfected cells and infected cells for the expression of each gene tested. The images represent some of the ISH reactions for the specified treatment condition and probe. Images were taken at 1000× magnification. U = uninfected cells, WT = infected cells, USTS = uninfected cells treated with staurosporine, and WTSTS = infected cells treated with STS. A: Induced genes. B: Repressed genes.