Distributions and means of ORF, 5' and 3' UTR sizes in reference FLcDNAs for (A) S. salar (B) E. lucius. Each reference FLcDNA, determined by in-house annotation methods, was examined for an ORF, 5' UTR, and 3' UTR. Means for each region were calculated (+/- standard deviation). An ORF is characterized by a start (ATG) and an in-frame stop codon (TGA, TAG, TAA). The 5' UTR is calculated as the entire area upstream of the start codon, while the 3' UTR is considered the entire area downstream of the stop codon. Any 3' polyA tails were masked and were not included in UTR length calculations.