Figure 9

Flowchart describing the computational pipeline used for prediction of novel miRNAs. The sequencing reads that did not match with any of the databases of elimination pipeline, but matched with the human intergenic and intronic sequences, were extracted along with flanking sequences from human genome. These were then analysed by a number of miRNA precursor prediction algorithms and the hits were further analysed by a set of filters as described. The final output of the pipeline gives a list of novel miRNAs.