Dissection of Hv sex pheromone gland for RNA extraction. A. Gland was forced out by squeezing the abdomen with pincet (the gland is similary inflated when the female calls (Groot, Schal, Gould, Classen, pers. obs). B. Gland was cut at the scleritozed cuticle from the 8th abdominal segment. C. Sclerotized cuticle was mostly removed before immersing gland in Trizol.