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Figure 2 | BMC Genomics

Figure 2

From: A 454 multiplex sequencing method for rapid and reliable genotyping of highly polymorphic genes in large-scale studies

Figure 2

Confidence level of genotyping. f is the probability of amplifying, at least three times, all the different variants of the gene studied for a given sample. This probability depends on n, the total number of sequences per sample, and m, the maximal number of variants for the gene within a sample. T 1 is the threshold value that corresponds to the minimal number of sequences required per individual to determine a complete genotype, with a 10-3 probability of missing a variant.

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