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Table 3 Genetic variation in exon 2 sequences of the DRB gene observed for 1,407 rodent samples from 24 species and 11 genera.

From: A 454 multiplex sequencing method for rapid and reliable genotyping of highly polymorphic genes in large-scale studies

Rodent Genera

Number of DRB copy amplified

Number of analysed samples

Replicates

Genotyped (p= 0.999)

Heterozygotes genotyped

Number of variant

Mean number of sequences per variant

   

Nb

%

Nb

%

Nb

%

  

Pool A

          

Myodes

2

650

33

5.1

602

93

495

82.2

71

834

Arvicola

1

49

2

4.1

49

100

36

73.5

13

324

Microtus

1

40

2

5.0

36

90

29

80.6

7

403

Apodemus

1

44

3

6.8

42

95

39

92.9

50

70

Pool B

          

Rattus

1

417

19

4.6

388

93

248

63.9

110

233

Mus

1

147

8

5.4

134

91

110

82.1

53

171

Niviventer

1

78

5

6.4

44

56

9

20.5

15

172

Bandicota

1

58

4

6.9

51

88

39

76.5

21

154

Berylmys

1

37

4

10.8

36

97

25

69.4

19

122

Leopoldamys

1

24

1

4.2

21

88

14

66.7

12

121

Maxomys

4

22

2

9.1

4

18

4

100.0

21

37

Total

 

1,566

83

5.3

1,407

90

1,048

74.5

392

277

  1. The confidence level used for validating genotypes was fixed at 10-3.