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Table 1 Performance of MLPA and MAQ

From: Multiplex Amplicon Quantification (MAQ), a fast and efficient method for the simultaneous detection of copy number alterations in neuroblastoma

  Chromosome 1p MYCN status Chromosome 3p Chromosome 11q Chromosome 17q
Segmental (n = 35) MLPA MAQ MLPA MAQ MLPA MAQ MLPA MAQ MLPA MAQ
AUC 94.4% 97.1% 100.0% 100.0% 94.3% 100.0% 97.1% 97.1% 100.0% 100.0%
Sensitivity 100.0% 94.4% 100.0% 100.0% 100.0% 100.0% 100.0% 100.0% 100.0% 100.0%
Specificity 88.9% 100.0% 100.0% 100.0% 92.0% 100.0% 95.5% 95.5% 100.0% 100.0%
PPV 90.0% 100.0% 100.0% 100.0% 83.3% 100.0% 92.9% 92.9% 100.0% 100.0%
NPV 100.0% 94.4% 100.0% 100.0% 100.0% 100.0% 100.0% 100.0% 100.0% 100.0%
  95% Confidence Interval AUC(MLPA): 94%-100% 95% Confidence Interval AUC(MAQ): 97%-100%
  Chromosome 1 Chromosome 2 Chromosome 3 Chromosome 11 Chromosome 17
Numerical (n = 17) MLPA MAQ MLPA MAQ MLPA MAQ MLPA MAQ MLPA MAQ
AUC 76.5% 88.2% 92.3% 76.9% 58.8% 76.5% 70.6% 76.5% 94.1% 94.1%
Sensitivity 77.8% 77.8% 90.0% 70.0% 46.2% 69.2% 71.4% 71.4% 94.1% 94.1%
Specificity 75.0% 100.0% 100.0% 100.0% 100.0% 100.0% 66.7% 100.0% 100.0% 100.0%
PPV 77.8% 100.0% 100.0% 100.0% 100.0% 100.0% 90.9% 100.0% 100.0% 100.0%
NPV 75.0% 80.0% 75.0% 50.0% 36.4% 50.0% 33.3% 42.9% 100.0% 100.0%
  95% Confidence Interval AUC(MLPA): 60%-97% 95% Confidence Interval AUC(MAQ): 72%-93%
  1. Performance in tumors with segmental (35) and numerical (17) aberrations for the recurrent NB copy number changes at chromosome 1(p), 2 (MYCN status), 3(p), 11(q) and 17(q). This evaluation includes area under curve (AUC), positive predictive value (PPV) and negative predictive value (NPV).