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Figure 3 | BMC Genomics

Figure 3

From: A simple, high throughput method to locate single copy sequences from Bacterial Artificial Chromosome (BAC) libraries using High Resolution Melt analysis

Figure 3

Screen of Matrix pools by MT-PCR-HRM. First order differential plots of a High Resolution Melt derived from a MT-PCR-HRM experiment for STS marker M106 (three replicates per treatment) performed on BAC matrix pools (5 × 384 well plates) containing a single target BAC (green line) or on the same matrix pool but lacking the target (pink line). All HRM analyses performed on a Qiagen Rotor-Gene 6000.

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