Skip to main content

Table 2 MT-PCR-HRM pooling strategy to accommodate for variable genome size.

From: A simple, high throughput method to locate single copy sequences from Bacterial Artificial Chromosome (BAC) libraries using High Resolution Melt analysis

Organism 1C genome (Mb) No 384 well plates No super pools No matrix pools No single pools Total required simplex MT-PCR-HRM Total required triplex MT-PCR-HRM
Brachypodium distachyon 300 24 1 10 54* 64 22
Potato 850 66 3 30 54 87 29
Barley 5500 430 17 30 54 101 35
Wheat 16000 1250 50 30 54 134 45
Swine 2600 203 8 30 54 92 31
Cattle 3100 242 10 30 54 94 32
  1. Estimated number of pools and MT-PCR-HRM reactions required to find a single marker (or 3 markers simultaneously) within the denoted genomes, assuming a 3× coverage and 100 Kb average insert size for the corresponding BAC libraries. Note: *1× coverage = 18 single pools (see Figure 1); 3× coverage = 54 single pools