Skip to main content

Table 2 MT-PCR-HRM pooling strategy to accommodate for variable genome size.

From: A simple, high throughput method to locate single copy sequences from Bacterial Artificial Chromosome (BAC) libraries using High Resolution Melt analysis

Organism

1C genome (Mb)

No 384 well plates

No super pools

No matrix pools

No single pools

Total required simplex MT-PCR-HRM

Total required triplex MT-PCR-HRM

Brachypodium distachyon

300

24

1

10

54*

64

22

Potato

850

66

3

30

54

87

29

Barley

5500

430

17

30

54

101

35

Wheat

16000

1250

50

30

54

134

45

Swine

2600

203

8

30

54

92

31

Cattle

3100

242

10

30

54

94

32

  1. Estimated number of pools and MT-PCR-HRM reactions required to find a single marker (or 3 markers simultaneously) within the denoted genomes, assuming a 3× coverage and 100 Kb average insert size for the corresponding BAC libraries. Note: *1× coverage = 18 single pools (see Figure 1); 3× coverage = 54 single pools